Journal: Journal of internal medicine

Article Title: Light transmittance in human atrial tissue and transthoracic illumination in rats support translatability of optogenetic cardioversion of atrial fibrillation.

doi: 10.1111/joim.13654

Figure Lengend Snippet: Fig. 2 Light penetration measurements in atrial tissue samples from patients to guide transthoracic optogenetic AF cardioversion in the intact adult rat. (a) fresh left atrial appendage excised from a patient during cardiac surgery; (b) tissue slices of the left atrial appendage cut with a vibratome for light penetration measurements; (c) measurements of 565-nm light penetrance in human atrial tissue slices of 0.5, 1.0, 1.5, and 2.0 mm (4 measurements per thickness of samples from 5 different patients is 80 measurements in total). Experimental data were fitted to a monoexponential decay function; (d) schematic drawing of the light-emitting diode (LED) device used for transthoracic illumination; (e) experimental setup of the transthoracic optogenetic AF cardioversion experiments; (f) quantification of transthoracic optogenetic AF termination in the red-activatable channelrhodopsin (ReaChR)-expressing intact adult rats after three consecutive 565-nm light pulses (25 mW/mm2) of 500 ms at 500-ms intervals, compared to Citrine-expressing control animals. Each dot indicates five termi- nation attempts in one rat (n = 4 rats for both groups). Error bar represents standard error of the mean (SEM). ***p < 0.001; (g) representative body surface electrocardiographic (ECG) trace showing 10-Hz transthoracic optical atrial pacing in the intact adult rat with 10-ms light pulses (565 nm, 25 mW/mm2); (h) representative body surface ECG demonstrating successful optogenetic cardioversion of AF by transthoracic illumination of the intact adult rat (500 ms, 25 mW/mm2).

Article Snippet: Bipolar electrodes were placed in the ventricles for ECG recording and connected to a PowerLab 15T acquisition device (AD Instruments) controlled by LabChart Pro software version 7.

Techniques: Expressing, Control

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Adult zebrafish ECG recording apparatus and protocol. (A) Comparison of human and zebrafish ECG. Top: ECG of a healthy 32-year-old human male, obtained from the MIT-BIH Normal Sinus Rhythm Database . Bottom: ECG from a 6-month-old adult male AB zebrafish, captured using the described recording apparatus and protocol. All cycles within the 60 s (human) and 120 s (zebrafish) recordings were overlaid (green traces), and an average trace was calculated (black trace) using the LabChart ‘ECG Analysis’ module. Traces are shown on the same time scale for direct comparison. (B) Adult zebrafish ECG apparatus; during recordings, the zebrafish is placed ventral side up within the mold located in a well on the base. For ease of reference, apparatus components and axes used to describe the movement of the components are labeled. (C) Summary of adult zebrafish ECG recording protocol. (D) Example from a 6-month-old adult male AB zebrafish using the described recording apparatus and protocol. The trace image was captured using LabChart ‘Zoom View’.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Comparison, Labeling

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Overview of zebrafish ECG reading GUI (zERG), a zebrafish ECG analysis software program. In step 1, ECG voltage measurements are imported into zERG. In step 2, data for peak analysis are selected. During peak analysis, P waves (red asterisks) and QRS complexes (black asterisks) are identified based on the following: (1) the user-defined wave amplitude threshold; (2) the minimum peak distance; and (3) classification based on the distance between the P peak to R wave of consecutive cycles and the reference distance defined as two times the first ‘P peak to R wave’ interval. Consecutive red or black peaks are marked yellow for error correction; the user can then manually add and/or delete P waves and/or QRS complexes. Once all waves are marked correctly, the user can proceed to step 3 by choosing the ‘Calculate Average Trace’ button. This plots an average trace and allows the user to click on ‘Add Markers’ to note wave markers on the average trace. Finally, after all peaks are confirmed, the user selects ‘Analyze ECGs’ to obtain a graphical user interface (GUI) table with the ECG metrics, in addition to an output .txt and several average trace plots. The yellow boxes show an example of a trace in which the P wave amplitude exceeds the R wave amplitude and how zERG handles such traces. The example trace in the blue boxes was captured from a 6-month-old AB wild-type male fish, and the trace in the yellow boxes was captured from a 3.5-month-old AB wild-type male fish after being dosed with 0.800 mmol/l FA for 15 min. All images were captured from zERG version 1.0.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Software

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Comparison of ECG metrics between zERG and LabChart. Measurements from a total of 55 traces from two independent experiments, either from 0.800 mmol/l FA drug studies ( n =14; referred to as FA) or from recording sessions in which 41 traces were captured from 11 AB fish over multiple timepoints (referred to as Timepoint), were used for comparison of zERG and LabChart performance. (A) Metrics obtained from zERG are nearly identical to those obtained from LabChart. (B) Comparison of heart rate between LabChart, zERG and a manual calculation. zERG heart rate calculations are nearly identical to those obtained from a manual calculation. bpm, beats/min.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Comparison

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Correlation of zebrafish ECG traits and covariates. Zebrafish ECG traits (heart rate and intervals) are correlated with each other and with body size parameters, sex and age. The correlation matrix is derived from measurements obtained from a total of 70 AB and wild-type fish from the kcnh6a s290 and kcnh6a tb218 lines. Positive correlations are colored in blue and negative correlations in red. The color intensity and size of the circles are proportional to the correlation (i.e. a correlation of 0.80 will have a larger and darker red circle than a correlation of 0.10).

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Derivative Assay

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Delayed ventricular repolarization in kcnh6a s290 /+ mutants. kcnh6a s290 /+ (heterozygous) mutants displayed a longer QT interval than their kcnh6a +/+ (wild-type) clutchmates ( P =0.021). Measurements were obtained from a total of 54 fish. The P -values were determined from a linear mixed model, in which the date of the ECG recording session was added as a random effect and age, ECG recording location, sex and weight were added as fixed effects; QT interval was additionally adjusted for heart rate. The QT interval plot shows the measurement uncorrected for heart rate.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques:

Journal:

Article Title: Cardiovascular Responses to Vibrostimulation for Sperm Retrieval in Men With Spinal Cord Injury

doi:

Figure Lengend Snippet: Arrhythmia and ECG Abnormalities Detected at Rest, During Vibrostimulation, Ejaculation, and Recovery *

Article Snippet: Subjects were instrumented with a standard 3-lead ECG (lead II; Powerlab Model ML132) and beat-to-beat blood pressure monitoring device (Finometer, Finapres Medical Systems BV, Arnhem, The Netherlands).

Techniques: